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. 2018 Nov 3;14(14):2023–2036. doi: 10.7150/ijbs.28302

Figure 5.

Figure 5

The CtBP2-p300-Runx2 transcriptional complex specifically binds to the Runx2 target gene promoters. (A) The CtBP2 was significantly enriched on the promoters of Runx2 target genes in AOB cells. The HOB-1 and AOB-3 cells were subjected to ChIP assays with CtBP1, CtBP2 and IgG antibodies, followed by analysis with qRT-PCR to evaluate their binding to the promoters of Runx2 target genes. CUL4A and FOXM1 were used as controls. **P<0.001. (B) The binding of p300 and Runx2 on the promoters of Runx2 target genes was dramatically decreased in AOB cells. The HOB-1 and AOB-3 cells were subjected to ChIP assays with p300, Runx2 and IgG antibodies, followed by analysis with qRT-PCR to evaluate their binding to the promoters of Runx2 target genes. CUL4A and FOXM1 were used as controls. **P<0.001. (C) The binding of Pol II on the promoters of Runx2 target genes was dramatically decreased in AOB cells. The HOB-1 and AOB-3 cells were transfected with control siRNA and CtBP2-siRNA and subjected to ChIP assays with Pol II and IgG antibodies, followed by analysis with qRT-PCR to evaluate their binding to the promoters of Runx2 target genes. CUL4A and FOXM1 were used as controls. **P<0.001.