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. 2018 Nov 3;14(14):2073–2082. doi: 10.7150/ijbs.28757

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The construction of TRIM59-shRNA-SKOV3 cell line. (A) Real-time PCR examined the expressions of TRIM59 mRNA in 7 ovarian epithelial cell lines. (B) The transfection efficiency of pUCTP-TRIM59-shRNA and pUCTP-scramble plasmids in SKOV3 cell line. (magnification ×200) (C) Real-time PCR identified the knockdown of TRIM59 in SKOV3 cell line. (D) Western blot identified the knockdown of TRIM59 in SKOV3 cell line. Results are displayed as means ± SD from experiments in triplicate. ***, P < 0.001.