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Histopathological examination flow chart: Prostates were put on ice to avoid mRNA degradation and transported to the pathologist immediately after radical prostatectomy. (1) One tissue sample designated for OSNA was extracted, snap-frozen and stored at -80°C. (2) OSNA analysis. (3) Formalin-fixed prostate tissue was embedded in paraffin and conventionally sectioned. (4) Slices were stained with hematoxylin and eosin (H&E) (4a) and CK19 immunohistochemistry (IHC) was performed (4b).
