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Lentiviral CRISPR/Cas9 nickase vector-mediated MTF1 gene editing resulted in inhibition of EMT in ovarian cancer cells. A. MTF1 and EMT marker expression was examined in MTF1 KO and control SKOV3 and OVCAR3 cells by Western blot. B. Immunofluorescent staining of MTF1 expression in SKOV3 MTF1 KO and control cells. C. Mesenchymal marker β-catenin was stained in SKOV3 MTF1 KO and control cells. D. Epithelial marker cytokeratin 7 was stained in SKOV3 MTF1 KO and control cells.
