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. 2018 Oct 20;15(14):1582–1590. doi: 10.7150/ijms.28452

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CD34 immunostaining. (a) Small blood vessels were observed in about one-third of the area from the entrance of the TCP holes. (b) Small blood vessels were observed up to the center of the TCP holes. (c) Angiogenesis was poor at 3 weeks compared with that at 2 weeks. (d) Microvessel invasion was also observed around the entrance of the TCP holes. (e) Vascular invasion was observed up to the center of the TCP holes, along with a branching network of blood vessel formation. (f) Blood vessels similar to sinusoidal vessels were observed in the bone marrow-like structures formed within the TCP holes. (g) A thick formation of vascular invasion was observed in the vicinity of the TCP hole entrances. (h) A plurality of thick rectilinear blood vessels penetrating into the central part of the TCP holes was observed. (i) At 3 weeks, a pattern of blood vessel invasion similar to that at 2 weeks was seen.