Table 1.
Antigen | Clone | Product code | Antibody class | Supplier | Dilution | Antigen retrieval |
---|---|---|---|---|---|---|
CK7 | Mouse monoclonal | NCL-CK7-OVTL | IgG1 | Leica | 1:200 | ER2 20 min |
CK19 | Mouse monoclonal | BA17 | IgG1 | ZETA | 1:100 | ER2 20 min |
CK8 | Mouse monoclonal | TA500021 | IgG2b | OriGene | 1:200 | ER2 20 min |
CK18 | Mouse monoclonal | TA500015 | IgG1 | OriGene | 1:200 | ER2 20 min |
SALL4 | Rabbit monoclonal | EP299 | IgG | ZETA | 1:200 | ER2 20 min |
ER1: Bond Epitope Retrieval Solution 1 contains a citrate-based buffer and surfactant. ER2: Bond Epitope Retrieval Solution 2 contains an ethylene diaminetetra-acetic acid-based buffer and surfactant.