Figure 2.

Expression profiling of chemokine receptors in cultured MSCs suggest genetically overexpressing CCR2. (A) The expression of CC and CXC chemokine receptors on fourth-passage (P4) MSCs were analyzed by qRT-PCR of mRNA samples from three independent donors. Surface markers including CD13, CD29, CD44, CD73, CD105, and CD90 were served as the positive control. n = 3. (B) The relative mRNA expression of CCR2 normalized to GAPDH was detected in MSCs from additional five independent donors by qRT-PCR analysis. (C) Flow cytometry analysis depicted that cultured MSCs (P4) were negative for CCR2 expression when compared with the positive control PBMCs. (D) MSCs with expression of CCR2 were isolated by FACS sorting. (E and F) CCR2 expression was confirmed by qRT-PCR (E) and western blotting (F). All data are expressed as means ± SEM; ***p < 0.001, and n.s. is non-significant.