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. 2018 Nov 12;8(21):5929–5944. doi: 10.7150/thno.28029

Figure 8.

Figure 8

MSCCCR2 restores BBB integrity via a PRDX4-mediated antioxidant mechanism. (A) RNA-seq data analysis of MSCs from three different donors. The heat map displayed genes encoding antioxidant proteins. This heat map was drawn with the RPKM value taking the logarithm of 2. Details of identified high-expressed genes were listed in the right table. (B) ELISA assay showed significant decrease of PRDX4 concentration in culture medium of MSCCCR2 treated with shPRDX4-1 and shPRDX4-2 respectively. n = 3. (C) ShPRDX4 treatment suppressed the antioxidant effects of MSCCCR2. (D) Transfection of shPRDX4 compromised the BBB preservative impacts of MSCCCR2 in vitro. Scale bars: 20μm. (E) The endothelial permeability was assessed by measuring the diffusion of sodium fluorescein. n = 5. (F) Representative immunostaining images of brain slices with BBB marker GLUT1. Scale bars: 75μm. All data are expressed as means ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, and n.s. is non-significant.