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. 2018 Nov 15;8(21):6025–6034. doi: 10.7150/thno.26607

Figure 2.

Figure 2

Absorption (Abs)/NIR II fluorescence /PA/PTT responses of IR1048-MZ to NTR in vitro with 500 μM NADH as a coenzyme. (A) Absorption spectra in the absence (black) and presence (blue) of NTR. (B) Fluorescence emission spectra (λexem = 980/1046 nm) in the absence (black) and presence (blue) of NTR; FI: fluorescence intensity. (C) Absorption responses of 5 μg/mL IR1048-MZ to different concentrations of NTR. Inset: a linear correlation between absorption intensity and concentration of NTR. (D) NIR II fluorescence responses of 5 μg/mL IR1048-MZ to different concentrations of NTR. Inset: a linear correlation between emission intensity and concentration of NTR. (E) PA responses of 5 μg/mL IR1048-MZ to different concentrations of NTR; PA Int.: PA intensity. Inset: PA imaging of 5 μg/mL IR1048-MZ solution with different concentrations of NTR. The impulse excitation wavelength was 880 nm. (F) PT responses of 5 μg/mL IR1048-MZ to different concentrations of NTR after 980 nm NIR laser irradiation (0.1 W/cm2) for 2 min. Inset: infrared thermal imaging of 5 μg/mL IR1048-MZ solution with different concentrations of NTR after NIR laser irradiation for 2 min.