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. 2018 Nov 15;8(21):5995–6007. doi: 10.7150/thno.27706

Figure 2.

Figure 2

The downregulation of CDC20 inhibited the phenylephrine (PE)-induced hypertrophic response in cultured cardiomyocytes. (A) Immunoblotting analysis of CDC20 in neonatal rat cardiomyocytes infected with adenovirus siCDC20 or siControl (n=3). (B) Representative images of double immunostaining (red for α-actinin, blue for DAPI) of NRCMs after 24 hours of PE (100 nM) (left). Scale bar: 50 μm. Quantification of the myocyte surface area (n=3, 150 cells counted per experiment; right). (C) qPCR analysis of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and β-myosin heavy chain (β-MHC) mRNA levels in NRCMs treated as described in (B) (n=3). (D) Immunoblot analysis of AKT, ERK1/2, p38 and GAPDH in NRCMs treated as described in (B) (n=3); * P < 0.05, ** P < 0.01 vs. control; # P < 0.05, ## P < 0.01 vs. Ad-siRNA-control plus PE.