Figure 6.

Knockdown of LC3 reversed the reduction of cardiomyocyte size after deletion of CDC20 in vitro. (A) Representative images of double immunostaining (green for α-actinin) of NRCMs transfected with siRNA-CDC20 or siRNA-control with or without Ad-siLC3 treatment after 24 hours of Ang II stimulation (left). Scale bar 50 μm. Quantification of the myocyte surface area (right) (n=3). (B) Representative Western blot analyses of p-AKT, AKT, p-ERK1/2, ERK1/2, NOX4, LC3 I/II and GAPDH in NRCMs infected with siRNA-control or siRNA-CDC20 with or without Ad-siLC3 treatment after 24 hours of Ang II stimulation (n=3). C, A functional link between CDC20 and LC3-dependent autophagy in hypertrophy. ** P < 0.01 vs. saline; ^# P < 0.05, ^## P < 0.01 vs. saline