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. 2018 Nov 12;8(21):5890–5902. doi: 10.7150/thno.27667

Figure 3.

Figure 3

BPI-9016M suppresses spreading, migration and invasion of lung adenocarcinoma. (A) Spreading ability assessed by the wound-healing assay of A549 and H1299 cells treated with BPI-9016M for 12, 24 and 36 h. (B) The spreading ability of A549 and 1299 cells treated with BPI-9016M, HGF or a combination of these two chemicals for 12, 24 and 36 h. (C) Representative images (×100 magnification) of migrated and invasive A549 and 1299 cells stained with crystal violet after treatment with vehicle or BPI-9016M. (D) The number of migrated and invasive cells treated with vehicle or BPI-9016M. (E) Representative images (×100 magnification) of migrated and invasive A549 and 1299 cells stained with crystal violet after treatment with HGF, BPI-9016M, or a combination of these two chemicals. (F) The number of migrated and invasive cells treated with HGF, BPI-9016M, or a combination of these two chemicals. (G) EMT-associated molecules including E-cadherin, N-cadherin and vimentin were assessed by Western blotting of A549 and 1299 cells after BPI-9016M treatment. (H) E-cadherin, N-cadherin and vimentin were detected by Western blotting in A549 and 1299 cells treated with HGF, BPI-9016M, or a combination of these two chemicals. (A-B, D, F) * p<0.05, ** p<0.001, *** p<0.0001.