Figure 3.
OKT11 (anti-CD2) and T3-3A1 (anti-CD7) IgG and F(ab´)2 show no functional T-cell impairment during short-term co-incubation. (A-B) PBMC-derived T cells were incubated for 18 h at 37 °C with various concentrations of IgG and F(ab´)2 in triplicates. Respective isotypes served as negative control, whereas OKT3 was used as positive control. Mean ± SD is depicted (n = 3). (A) Percentage of apoptotic PBMC-derived T cells after co-incubation at indicated protein concentrations. Values are based on flow cytometry analysis identifying annexin V-APC or 7-AAD positive cells of all cells detected. (B) Corresponding IFNγ release of PBMC-derived T cells in supernatants after incubation with indicated concentrations of IgG or F(ab´)2 determined by ELISA. (C) CTV-labeled PBMC-derived T cells were incubated with 100 nM of the indicated antibodies or F(ab´)2 at 37 °C over a period of 3 days. Incubation with isotypes served as negative control and OKT3 as positive control. Decrease of MFI of CTV indicates T-cell proliferation in response to indicated IgG or F(ab´)2 depicted as mean ± SD from triplicates (n = 3).