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. 2018 Nov 28;8(21):6070–6087. doi: 10.7150/thno.27275

Figure 4.

Figure 4

T3-3A1 (anti-CD7) IgG and F(ab´)2 have no impact on the cytotoxic efficacy of specifically activated TCR2.5D6iRFP TCM in contrast to co-incubation with OKT11 (anti-CD2) resulting in minor changes in tumor cells killing and IFNγ secretion. TCR2.5D6iRFP TCM were co-cultured with tumor cells bearing either the respective restriction element HLA-B7 or the irrelevant HLA-B15 for 24 h at 37 °C. As control, iRFP TCM were co-incubated with ML2-B7 tumor cells. Each condition is indicated below the graph. After incubation of the co-culture with respective IgG or F(ab´)2, cells were harvested for flow cytometric analysis and IFNɣ secretion in supernatants was analyzed by ELISA. The experiment was performed in triplicates and results are shown as mean ± SD (n = 3). One representative experiment out of three is depicted. Statistically significant differences were observed in single but not all experiments for full IgG and F(ab´)2. (A) GFP+ tumor cells per microliter calculated from flow cytometric analysis. (B) Corresponding IFNγ cytokine secretion in co-culture supernatant.