Figure 1.

Microfluidic perfusion device for engineering 3D bone tissues. (a) Actual device, containing bone tissue constructs in the central chamber with medium flowing into one inlet fed by a syringe pump and exiting through two outlets carrying effluent to a collection vial. Tissues were constructed using BCP microbeads and primary human osteocytes assembled at a 1:1 ratio (b) Harvested 3D tissue. (c) Schematic illustration of the tissue constructs showing the location of the PCa cells, osteoblasts forming the endosteum, and the spatial distribution of osteocytes and microbeads (not drawn to scale).