TABLE 1.

Phosphopeptides identified in the wild-type and MAPK mutant upon exposure or nonexposure to Congo red

Condition	PRa	Phosphopeptide	Abundance					Ratio	P value	p38 MAPK activity (AU)c
			WT		ΔmpkC		T0 ΔsakA ΔmpkC
			T0b	CR	CR	T0
CR
WT	S24	ISEEEEYEVTSPTDPTFR	3e+07	1.1e+08	NId	NI	NI	WT+CR/T0 WT = 3.37	0.009	0.484
	S39	DDKYSPIQK	NI	1.9 + 06	NI	NI	NI	Identified only with CR	0.02	0.532
ΔmpkC	S24	ISEEEEYEVTSPTDPTFR	NI	1.1e+08	4.5e+07	NI	NI	ΔmpkC+CR/WT+ CR = 0.41	0.03	0.484
T0
ΔmpkC	S24	ISEEEEYEVTSPTDPTFR	3e+07	NI	NI	9.2 + 07	NI	T0 ΔmpkC/T0 WT = 2.83	0.04	0.484
ΔsakA ΔmpkC	S24	ISEEEEYEVTSPTDPTFR	1e+06	NI	NI	NI	3e+07	T0 ΔsakA ΔmpkC/T0 WT = 27.5	0.002	0.484

^aPR, phosphoresidue.

^bT₀, time zero.

^cPredicted by NetPhos (http://www.cbs.dtu.dk/services/NetPhos-3.1/).

^dNI, not identified.