FIG 2.

Altering viral capping efficiency negatively impacts viral infection. (A) Multi-step growth kinetics of the individual capping mutants and parental wild-type SINV as observed in BHK-21 cells infected at an MOI of 0.5 PFU/cell. Statistical significance was determined by analysis of the area under the curve. (B) Plaque morphology of wild-type SINV and capping mutant viruses in BHK-21 cells overlaid with 0.5% solution of Avicel at 24 h post-infection. (C) Graph indicating the average plaque diameter of the mutant viruses relative to wild-type SINV. Size was determined by ImageJ software (NIH). (D) Cell viability of BHK-21 cells infected with the individual capping mutants at 24 hpi relative to mock-infected BHK-21 cells. All quantitative data shown represent means of results from at least three independent biological replicates, with error bars representing standard deviations of the means. Statistical significance, as indicated within each panel, was determined by Student's t test.