TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant property(ies)a | Reference or source |
|---|---|---|
| D. discoideum Ax3 | Parental strain | 59 |
| E. coli TOP10 | Invitrogen | |
| L. pneumophila GS3011 | L. pneumophila JR32 icmT3011::Kanr (ΔicmT) | 60 |
| L. pneumophila JR32 | Virulent L. pneumophila serogroup 1 strain Philadelphia | 61 |
| L. pneumophila CR02 | JR32 ralF::Kanr (ΔralF) | 30 |
| Plasmids | ||
| pDM317 | Dictyostelium extrachromosomal expression vector, N-terminal GFP, G418r | 62 |
| pDM323 | Dictyostelium extrachromosomal expression vector, C-terminal GFP, G418r | 62 |
| pDM1044 | Dictyostelium extrachromosomal expression vector, C-terminal mCherry, Hygr | 63 |
| pDXA-HC | Dictyostelium expression vector, Pact15, Neor, Ampr | 64 |
| pGolvesin-GFP | Full-length gene encoding D. discoideum golvesin | 37 |
| pHKB95 | pDM317-gfp-2×FYVE | H. Koliwer-Brandl et al., submitted for publication |
| pNP099 | pMMB207-C, ΔlacIq (constitutive mCerulean), Camr | 19 |
| pNP102 | pMMB207-C, ΔlacIq (constitutive mCherry), Camr | 19 |
| pPH_FAPP1 | pEGFP-N1-PHFAPP1-mCherry | 36 (gift of A. Helenius) |
| pRM010 | pSW102-PHFAPP1-gfp, G418r | This work |
| pSE002 | pDXA-2×PHFAPP1-gfp, G418r | This work |
| pSW102 | pDXA-MCS-gfp, G418r | 57 |
| pWS032 | pDM1044-P4CSidC-mCherry | 19 |
| pWS033 | pDM323-2×PHFAPP1-gfp | This work |
| pWS034 | pDM323-P4CSidC-gfp | 58 |
| pWS035 | pDM1044-2×PHFAPP1-mCherry | This work |
| pWS036 | pDM323-Arf1-gfp | This work |
| pWS038 | pDM323-Δ(1–75;119–579)golvesin-gfp | This work |
a
Abbreviations: Amp, ampicillin; Cam, chloramphenicol; Hyg, hygromycin; Kan, kanamycin; G418, Geneticin.