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. 2018 Dec 13;23(50):1800228. doi: 10.2807/1560-7917.ES.2018.23.50.1800228

Table 2. Description of chikungunya and dengue virus positive samples by real-time reverse transcription-PCR and corresponding Nanopore sequencing data, United Kingdom, 2017a (n = 8 samples).

Sample Ct value cDNA amount used for the library (ng) Sequencing kit
(2D kit version)
Flow cell
(FLO-)
1D total bp 1D total
reads
1D mean
read length (nt)
1D max
read length (nt)
CHIKV 1 14.7 431.5 SQK-NSK007 MIN105 1.51E + 08 267,171 564 92,712
CHIKV 3 21.4 928.8 SQK-LSK208 MIN106 1.63E + 09 1,891,028 862 99,031
CHIKV 4 22.7 113.4 SQK-NSK007 MIN105 1.74E + 08 216,493 805 125,387
CHIKV 9 30.1 212.4 SQK-LSK208 MIN106 2.12E + 09 3,481,358 608 121,711
DENV 1 16.3 1,626.0 SQK-NSK007 MIN105 2.42E + 08 284,622 851 115,494
DENV 2 16.9 1,626.0 SQK-NSK007 MIN105 1.55E + 08 203,700 760 52,157
DENV 6 21.2 475.0 SQK-LSK208 MIN106 1.22E + 09 1,377,721 886 118,733
DENV 11 28.7 65.8 SQK-LSK208 MIN106 7.07E + 08 1,111,566 636 119,438

Ct: cycle threshold.

a The Nanopore data presented in the table were obtained in 2017 on samples that had been collected and found positive for chikungunya or dengue virus by real-time reverse transcription-PCR in 2016.

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