Table 1.
Putative proteins within the calbistrin cluster in P. decumbens
| Name | P. decumbens locus | Size (aa) | BLASTP Aver |
%I | BLASTP Aacu |
%I | Conserved domain and notes | E-value |
|---|---|---|---|---|---|---|---|---|
| CalA | PENDEC_c013G00595 | 2910 | OJJ08178.1 | 85.3 | XP_020058113.1 | 78.1 | PKS: KS, AT, ACP, DH, Cmet, KR, R Note: similar to MlcA PKS |
0.0 |
| CalB | PENDEC_c013G07044 | 562 | OJJ08177.1 | 84.9 | XP_020058136.1 | 79.4 | TIGR00711, drug resistance transporter, Note: similar to MlcE MFS pump |
4.1E−40 |
| CalC | PENDEC_c013G06298 | 426 | OJJ08176.1 | 74.6 | XP_020058137.1 | 51.9 | smart00066, GAL4-like Zn(II)2Cys6 DNA-binding domain | 3.3E−05 |
| CalD | PENDEC_c013G04601 | 494 | OJJ08174.1 | 89.6 | XP_020058121.1 | 76.1 | No putative conserved domains detected. | |
| CalE | PENDEC_c013G04259 | 494 | OJJ08173.1 | 77.2 | XP_020058122.1 | 49.8 | pfam00067, Cytochrome P450 Note: similarity to MlcC monooxygenase |
1.8E−36 |
| CalF | PENDEC_c013G03789 | 575 | OJJ08172.1 | 85.4 | XP_020058123.1 | 75.4 | COG0277, FAD/FMN-containing dehydrogenase Note: similar to the bifunctional Sol5 flavin-dependent oxidase and alderase from Alternaria solani |
2.7E−22 |
| CalG | n/a | 1056 | OJJ08171.1 | 84.9 | XP_020058124.1 | 72.8 | pfam00067, Cytochrome P450, + CYPOR Bifunctional: N-term cytochrome P450 and C-term cytochrome P450 reductase domains |
2.5E−78 |
| CalH | PENDEC_c013G02261 | 273 | OJJ08170.1 | 82.6 | XP_020058125.1 | 61.2 | pfam08242, SAM dependent methyltransferase Note: similarity to C-MET domain found in HR-PKSs: FUM1, EasB, LepA, ApdA and AzaB |
1.6E−20 |
| CalI | PENDEC_c013G00477 | 383 | OJJ08169.1 | 82.7 | XP_020058126.1 | 72.4 | PRK06196, oxidoreductase (dehydrogenase) | 1.1E−75 |
| CalJ | n/a | 418 | OJJ08168.1 | 82.1 | XP_020058127.1 | 68.5 | pfam00144, Beta-lactamase (putative acyltransferase) Note: similar to MlcH acyltransferase |
1.7E−33 |
| CalK | PENDEC_c013G03312 | 194 | OJJ08167.1 | 83.0 | XP_020058128.1 | 67.0 | cd08249, enoyl reductase like Note: similar to MlcG ER |
3.5E−110 |
| CalL | PENDEC_c013G00617 | 568 | OJJ08166.1 | 88.1 | XP_020058129.1 | 78.2 | pfam00067, Cytochrome P450 | 5.7E−23 |
| CalM | n/a | 304 | OJJ08165.1 | 89.8 | XP_020058138.1 | 78.3 | PRK06180, short chain dehydrogenase | 1.5E−67 |
The gene names calA–calM were defined in this study. The PENDEC_XXXXX accession numbers are as in the original publication of the genome, except for calG, calJ and calM. These new gene models were constructed using Softberry FGENESH supported with homologous genes in A. versicolor (Aver) and A. aculeatus (Aacu) (see Additional file 1: additional information 16 for protein sequences of P. decumbens CalG, CalJ and CalM proteins). Putative homologues of each of the P. decumbens CAL protein in A. aculeatus and A. versicolor were identify by BLASTP are here presented with accession umber, along with % identity at amino acid level (%I) along with the predicted conserved domains found in the protein and E-value for this prediction