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. 2018 Dec 18;18:217. doi: 10.1186/s12866-018-1346-x

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© The Author(s). 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Analysis of the recombinant proteins VirB9-1, VirB9-2 and VirB10. SDS-PAGE and Western Blot analysis of recombinant VirB9-1 (a), VirB9-2 (b), and VirB10 (c). Lanes represent the different fractions analyzed during the purification procedure, 1 = Total crude protein, 2 = Filtered supernatant fraction obtained after high-speed centrifugation, 3 = Washed fraction, 4 = Eluted protein. Western Blot analysis was performed using the monoclonal anti-histidine tag antibody reacting with the recombinant protein (red arrows). Predicted molecular weights plus tags for VirB9-1 (33.5KDa), VirB9-2 (31.6KDa) and VirB10 (52KDa)