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. 2018 Dec 18;13:32. doi: 10.1186/s12263-018-0623-5

Table 1.

Primer sequence and the annealing temperature used for methylation-specific PCR

Gene Forward primer Reverse primer Annealing T ( °C)
miR-126 U: 5′-GTGGTGGTGGTGTGTGTGTGTTT-3′ 5′-CTCAACCCAACCCAAACAACAACCA-3′ 60
M: 5′-GCGGCGCGTGCGCGTTT-3′ 5′-CCAACCCGAACGACGACCG-3′