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. 2018 Dec 18;13:32. doi: 10.1186/s12263-018-0623-5

Table 2.

Primers’ sequence used for quantitative real-time RT-PCR

Gene Forward primer Reverse primer Annealing T (°C)
VEGF 5′-TGCAGATTATGCGGATCAAACC-3′ 5′-TGCATTCACATTTGTTGTGCTGTAG-3′ 60
GAPDH 5′-CGACCACTTTGTCAAGCTCA-3′ 5′-AGGGGTCTACATGGCAACTG-3′ 60