Fig. 1.

PD1-Fc-OX40L ARC retains proper folding and binding to PD-L1 and OX40. a Predicted tertiary structure of human PD1-Fc-OX40L. b Western blot analysis of PD1-Fc-OX40L performed by probing purified protein with human anti-PD1, anti-Fc, and anti-OX40L, under non-reducing and reducing conditions, and ± the deglycosylase PNGase F. c Functional ELISA using capture with recombinant hPD-L1 followed by detection with recombinant hOX40-His and then anti-His-HRP. HVEM-His serves as a negative control. d Functional PD-L1 blocking assay testing the ability of hPD1-Fc-OX40L to outcompete PD1-Biotin for binding to plate bound PD-L1 in an ELISA format. Avidin-HRP was used for signal detection