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. 2018 Dec 18;12:34. doi: 10.1186/s13036-018-0128-1

Fig. 3.

Fig. 3

Cultivation of U. maydis AB33P5ΔR/AtPgaX with varying galacturonic acid and buffer concentrations. The medium was supplemented with glucose (4 g/L) and varying amounts of initial galacturonic acid (GalA0) and MOPS buffer. 0, 4.5, 8.8, 12.8, 16.9, 21.7, 26.1 and 34.6 g/L GalA0 was supplemented with 0.1, 0.1, 0.1, 0.15, 0.2, 0.25, 0.3 and 0.4 M MOPS, respectively. a Oxygen transfer rate. The galacturonic acid consumption phase is indicated by the shaded area below the OTR curve for each cultivation. b Dashed lines represent the predicted residual substrate concentration in the culture medium (GalAcalc) according to Eq. (6). Symbols represent offline measured values (GalAmeas). Culture conditions: modified Verduyn medium, initial pH 6.5, 250 mL flask, filling volume 20 mL, shaking frequency 300 rpm, shaking diameter 50 mm, initial OD600 = 0.65, T = 30 °C