Fig. 5.

Cultivation of U. maydis AB33P5ΔR and AB33P5ΔR/AtPgaX on (poly)galacturonic acid. The strains were grown on glucose (4 g/L) and galacturonic acid or polygalacturonic acid (20 g/L) as carbon sources. a Mean value of duplicates of oxygen transfer rate. b Mean value of duplicates of respiratory quotient. The dotted horizontal line represents RQ = 1. c Predicted residual substrate concentration in the culture medium (GalA_calc or PolyGalA_calc) according to Eq. (6). The RQ drops below 1 after 40 and 44 h, respectively, indicating depletion of galacturonic acid. Thus, the line for GalA_calc in Fig. 5c is dotted from that time point on. Symbols represent offline-measured values. The mean values of biological duplicates are shown for online measurements for better clarity. Culture conditions: modified Verduyn medium, 0.2 M MOPS, initial pH 6.5, 250 mL flask, filling volume 20 mL, shaking frequency 300 rpm, shaking diameter 50 mm, initial OD₆₀₀ = 0.2, temperature 30 °C