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. 2018 Dec 18;12:34. doi: 10.1186/s13036-018-0128-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Comparison of enzymatic activity determined from offline (DNS) and online (OTR) measurements. The hydrolytic enzymatic activity on polygalacturonic acid was determined by offline DNS assays (blue bars) or by online determination of the linear substrate consumption shown in Fig. 6 (red bars). Samples of culture supernatant were taken and analysed after cultivation times of 17 and 23 h. For offline assays, the reaction time was 4 h and the polygalacturonic acid concentration 4 g/L. Light blue bars show the galacturonic acid concentration at the beginning of the offline assay