Figure 4. Single and combined treatment with As₂O₃ and resveratrol reduced the expression of B-cell lymphoma 2 (BCL2) family proteins. SK-N-SH cells were untreated, or treated with 75 μg/ml resveratrol alone, 2 μM As₂O₃ alone or As₂O₃ plus resveratrol for 48 h. Equal amounts of protein lysates from each sample were subjected to 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride membranes, and subsequently immunoblotted with anti-BCL2, anti-BH3 interacting domain death agonist (BID), anti-B-cell lymphoma (BCL)-x/L, and anti-β-actin. The signals were visualized via enhanced chemiluminescence and quantitated with the Image J software. Results represent the mean±S.D. of three independent experiments. *Significantly different at p<0.05.