Figure 5. Single and combined treatment with As₂O₃ (As) and resveratrol (Res) altered the expression of apoptosis-related caspases. SK-N-SH cells were treated with 75 μg/ml resveratrol, 2 μM of As₂O₃ or drugs combined for 48 h. Equal amounts of protein lysates from each sample were subjected to 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) together with the protein markers, transferred onto polyvinylidene fluoride membranes, and subsequently blotted with specific anti-procaspase-3, anti-procaspase-9, anti-cleaved caspase-3, anticleaved caspase-9 and anti-β-actin antibodies for 2 h. The final signals for each specific protein on the same membranes were visualized by enhanced chemiluminescence kit and quantitated with the Image J software. Results represent the mean±S.D. of three independent experiments. *Significantly different at p<0.05.