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. 2015 Feb 26;13(1):59–64. doi: 10.1016/j.jgeb.2015.01.002

Table 2.

Effect of different metal ions (5 mM), detergents and oxidizing agents (1%) on the activity of protease from Bacillus sp. after 30 min at 37 °C.

Enzyme activity (U/mg) Relative activity (%)
Metal ions (5 mM)
Ca2+ 9660 140
Mn2+ 8970 130
Mg2+ 5520 80
Zn2+ 5175 75
Ba2+ 5106 74
Cu2+ 3450 50
Co2+ 3105 45
Hg2+ 2070 30
Control 6900 100



Detergents and oxidizing agents (1%)
Tween-80 5520 80
SDS 5175 75
Triton-X-100 4140 60
Hydrogen peroxide 3105 45
Control 6900 100