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. Author manuscript; available in PMC: 2019 Dec 11.
Published in final edited form as: Chem Commun (Camb). 2018 Dec 11;54(99):14005–14008. doi: 10.1039/c8cc07432a

Fig. 4.

Fig. 4.

Genetic encoding of AsphK in mammalian cells. (a) Structural diagram of the mCherry-TAG-EGFP-HA reporter. (b) Bright field and fluorescent micrographs of HEK 293T cells transfected with plasmids encoding mCherry-TAG-EGFP-HA and TCOKRS/tRNAPyl CUA and cultured in DMEM medium supplemented with 10% FBS in the absence or presence of 1 mM AsphK. Scale bar = 1000 μm. (c) Western blot analysis of HEK 293T cell lysates probed with anti-HA antibody.