Table 5.
Primers used in ISSR analysis, their sequences, AB, PB,%P, PIC, MI and FS for wheat lines (L1–L20).
| Primer | Sequence | AB | PB | %P | PIC | MI | FS (bp) |
|
|---|---|---|---|---|---|---|---|---|
| Larger | Smaller | |||||||
| UBC-808 | (AG)8C | 6 | 2 | 33.3 | 0.06 | 0.12 | 940 | 560 |
| UBC-811 | (GA)8AC | 8 | 5 | 62.5 | 0.29 | 1.45 | 1200 | 440 |
| UBC-812 | (GA)2GG(AG)4AA | 5 | 1 | 20.0 | 0.04 | 0.04 | 1000 | 775 |
| UBC-815 | (TC)8A | 5 | 1 | 20.0 | 0.04 | 0.04 | 920 | 450 |
| UBC 818 | G(GGGGT)3 | 7 | 3 | 42.9 | 0.10 | 0.30 | 1635 | 620 |
| UBC-819 | (GT)8A | 5 | 1 | 20.0 | 0.04 | 0.04 | 1200 | 725 |
| UBC-826 | (AC)8C | 6 | 2 | 33.3 | 0.06 | 0.12 | 1370 | 535 |
| UBC-833 | (GA)8TT | 11 | 5 | 45.5 | 0.10 | 0.50 | 2000 | 635 |
| UBC-834 | (AG)8YT | 7 | 2 | 28.6 | 0.13 | 0.26 | 1850 | 880 |
| UBC-840 | (CT)8TT | 12 | 7 | 58.3 | 0.23 | 1.61 | 1950 | 725 |
| UBC-846 | (CA)8RT | 9 | 1 | 11.1 | 0.04 | 0.04 | 1000 | 530 |
| UBC-849 | (GT)8YA | 8 | 2 | 25.0 | 0.06 | 0.12 | 1025 | 590 |
| UBC-852 | (GATA)2(GACA)2 | 5 | 1 | 20.0 | 0.04 | 0.04 | 1345 | 420 |
| UBC-876 | (GATA)2(GACA)2 | 7 | 3 | 42.9 | 0.10 | 0.30 | 1840 | 770 |
| UBC-880 | (TC)8AA | 4 | 1 | 25.0 | 0.12 | 0.12 | 1250 | 550 |
| UBC-881 | (GGGTG)3 | 12 | 9 | 75.0 | 0.20 | 1.80 | 1485 | 535 |
| Total | 117 | 46 | ||||||
| Means | 7.3 | 2.9 | 0.10 | 0.43 | ||||
AB, amplified bands; PB, polymorphic bands;%P, percent of polymorphism; PIC, polymorphism information content; MI, marker index; FS, fragment size and bp, base pair.