. 2015 Nov 3;13(2):101–109. doi: 10.1016/j.jgeb.2015.10.001

Table 1.

Primers sequences used in PCR screening of the tested Cry-genes.

Primer	Sequence 5′ → 3′	Product size	Annealing temp. (°C)	References
Cry1,Un1(d)	CATGATTCATGCGGCAGATAAAC	274–277 bp	55	[12]
Cry1,Un1(r)	TTGTGACACTTCTGCTTCCCATT	274–277 bp	55	[12]
Cry1B CJ8	CTTCATCACGATGGAGTAA	367 bp	46	[51]
Cry1B CJ9	CATAATTTGGTCGTTCTGTT	367 bp	46	[51]
Cry1c CJ10	AAAGATCTGGAACACCTTT	130 bp	45	[51]
Cry1c CJ11	CAAACTCTAAATCCTTTCAC	130 bp	45	[51]
Cry2,Un2(d)	GTTATTCTTAATGCAGATGAATGGG	689–701 bp	55	[12]
Cry2,Un2(r)	CGGATAAAATAATCTGGGAAATAGT	689–701 bp	55	[12]
Cry2Aa,b(d)	CAG ATA CCC TTG CTC GTG TAA	1072 bp	54	[52]
Cry2Aa,b(r)	ATA GGC CCG TGC TCC ACC AGG	1072 bp	54	[52]
Cry3,Un3(d)	CGTTATCGCAGAGAGATGACATTAAC	589–604 bp	55	[12]
Cry3,Un3(r)	CATCTGTTGTTTCTGGAGGCAAT	589–604 bp	55	[12]
Cry4,Un4(d)	GCATATGATGTAGCGAAACAAGCC	439 bp	60	[12]
Cry4,Un4(r)	GCGTGACATACCCATTTCCAGGTCC	439 bp	60	[12]
Vip3Aa1(d)	ATGAACAAGAATAATACTAAATTAAGC	Varied according to subspecies	54	[53]
Vip3Aa1(r)	GGTCGACTTACTTAATAGAGACATCG	Varied according to subspecies	54	[53]