Table 4.
Effect of metal ions on laccase activity.
| Metal ion | Relative activity [% of control] |
50 mM | ||
|---|---|---|---|---|
| 6.25 mM | 12.5 mM | 25.0 mM | ||
| Mg2+ | 107 ± 2.9 | 87.3 ± 0.9 | 64.6 ± 13.5 | 55.2 ± 5.6 |
| Mn2+ | 87.3 ± 4.2 | 54.2 ± 4.2 | 44.5 ± 2.4 | 29.4 ± 1.1 |
| Ca2+ | 76.9 ± 18.8 | 54.9 ± 5.5 | 29.7 ± 1.4 | 22.1 ± 5.3 |
| Ni2+ | 60.0 ± 12.0 | 46.9 ± 2.8 | 24.9 ± 5.0 | 13.0 ± 1.1 |
| Co2+ | 59.1 ± 4.9 | 38.8 ± 3.8 | 29.1 ± 3.3 | 13.0 ± 2.6 |
Assays were carried out in final concentrations of 6.25–50 mM of chloride salts of Magnesium, Manganese, Calcium, Nickel and Cobalt. The relative activity was determined by measuring the laccase activity in the control that contained no chloride salts. The values shown represent the average from triplicate experiments. Error bars represent the standard deviation.