Figure 7.
Gene specific PCR analysis for the detection of At-NPR1 cotton transgenic event from genomic DNA isolated from leaves of to putative cotton transformants of LRK-516. Lane M, 1 kb ladder; Lane 1–2, At-NPR1 cotton transgenic samples; Lane 3, positive control of Arabidopsis thaliana sample; Lane 4, positive control of pBinAR-At-NPR1 plasmid sample; Lane 5, negative control of wild type G. hirsutum cv LRK-516 (Anjali) sample; Lane 6, negative control of pBinAR plasmid; and Lane 7, PCR negative control .