Table 1.
Percentage germination, survival rate, transformation frequency and molecular characterization of transgenic cotton G. hirsutum cv LRK-516 (Anjali) developed by leaf bisection method of In-planta Agrobacterium mediated transformation.
| Sr. No. | Number of seeds inoculated for germination | Number of seeds germinated | % of germination | Number of plantlets survived after treatment | % of survival | Number of PCR positive plantlets | % of transformation efficiency |
|---|---|---|---|---|---|---|---|
| 1 | 30 | 28 | 93.3 | 08 | 32 | 0 | 00.00 |
| 2 | 30 | 29 | 96.6 | 11 | 34 | 1 | 09.09 |
| 3 | 30 | 25 | 83.3 | 10 | 40 | 1 | 10.00 |
| Total | 90 | 82 | 91.11 | 29 | 35.36 | 2 | 06.89 |