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. 2016 May 18;14(1):9–18. doi: 10.1016/j.jgeb.2016.05.003

Table 2.

Details of primers used in transgenic event characterization.

Sr. No. Primer ID Primer sequence (5′-3′) Target
1 NPR1-F CTTGGCTCTGCTCGTCAATGG Arabidopsis thaliana ecotype Col-0, NPR1 gene
NPR1-R GGATGCAAAACGAAGAGCGA



2 nptII-F AGATCCCGTGGGCGAAGAA Neomycin phosphotransferase (nptII) gene from pBinAR
nptII-R GTACTCGGATGGAAGCCGGT



3 VB-LB-F GCTCTGCTAGGTAGCCCGATACGAT Left border + Ti-plasmid
VB-LB-R TATCCTGCCACCAGCCAGCCAACAG



4 VB-RB-F TTGGCGGGTAAACCTAAGAG Right border + Ti-plasmid
VB-RB-R TCGTCGAAGGCGTCTATCG



5 oriV-F ATAAGTGCCCTGCGGTATTG oriV in Ti-plasmid
oriV-R GCAGCCCTGGTTAAAAACAA