Table 4.

Composition of PCR cocktail prepared.

Sr. No.	Components	Volume (μl)
1	Template DNA (25 μg/μl)	2.0
2	Forward primer (10 mM)	1.0
3	Reverse primer (10 mM)	1.0
4	Taq DNA polymerase (5 U/μl)	0.3
5	dNTPs (25 mM)	0.5
6	10× PCR buffer	5.0
7	MgCL2 (15 mM)	1.5
8	Sterile double distilled water	38.7
	Total	50.0

All the additions and reaction setup must be done at 4 °C.