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. 2018 Dec 12;9:2940. doi: 10.3389/fimmu.2018.02940

Figure 1.

Figure 1

Flow cytometric characterization of PBMCs, tCD19, and NKG2D-CAR T cells in a representative NKR-2 T cells process. Mononuclear cells purified from blood using density gradient method were analyzed before (PBMCs) and after (tCD19 and NKR-2 T cells) the NKR-2 T cells process. After acquisition, cells were gated on SSC/FSC for Lymphocytes. Lymphocytes were then gated on CD3. All CD3 positive cells were then displayed as followed: (A) CD4/CD8 distribution. (B) Surface expression of NKG2D (CD314). (C) Memory phenotype. CD62L and CD45RA for the distinction of Naïve (CD62L+ CD45RA+), central memory (CD62L+ CD45RA–), effector memory (CD62L– CD45RA–), and terminal differentiated T cells expressing CD45RA (CD62L– CD45RA+) and (D) Exhaustion phenotype as stained for CD223 (Lag-3) and CD279 (PD-1). One representative donor out of 3 is shown.