Figure 1.

AGXX® has a strong antimicrobial effect on S. aureus USA300. (A) Growth curves of S. aureus USA300 were performed in RPMI medium which was exposed to 3–5 μg/ml AGXX® stress at an OD₅₀₀ of 0.5. The results are from 3 biological replicate experiments. Error bars represent the standard deviation (SD). (B) Viability of S. aureus USA300 wild type was analyzed before and 30 min after exposure to sub-lethal (4 μg/ml) or lethal amounts (10 μg/ml) of AGXX® at OD₅₀₀ of 0.5 using the LIVE/DEAD™ BacLight™ Bacterial Viability Kit. Live and dead S. aureus USA30 cells were visualized with a fluorescence microscope (Nikon, Eclipse, Ti2). S. aureus with an intact cytoplasmic membrane shows green fluorescence and dead cells with damaged membranes exhibit red fluorescence. The black arrow denotes AGXX® microparticles.