Figure 5.

BSH protects S. aureus against AGXX® stress. Growth phenotype analyses (A–C) and survival assays (D–E) were performed for the S. aureus USA300 wild type (WT) and the bshA mutant before and after exposure to AGXX® stress. (A–C) For growth comparisons, strains were exposed to 3, 4, and 5 μg/ml AGXX® stress at an OD₅₀₀ of 0.5. The results are from 3 biological replicate experiments. Error bars represent the SD and the statistics was calculated using a Student's unpaired two-tailed t-test by the graph prism software. Symbols are defined as follows:*p ≤ 0.05; **p ≤ 0.01 and ****p ≤ 0.0001. (D–E) For survival assays, S. aureus USA300 wild-type (D) and the bshA mutant (E) were grown in RPMI until an OD₅₀₀ of 0.5 and treated with 2–10 μg/ml AGXX®. Survival assays were performed by spotting 10 μl of serial dilutions after 2 and 3 h of AGXX® exposure onto LB agar plates to observe CFUs after 24 h incubation.