Figure 3.

Quantitative real-time PCR for the endogenous pluripotency markers pOCT4, pSOX2, pLIN28 and pNANOG was performed in the four piPSC lines at passage 10 and 20. Relative expression is shown as the fold change (calculated using 2 ^−ΔΔCT). The result showed the expression of endogenous genes pOCT4, pSOX2, pLIN28 and pNANOG were up-regulated in the four piPSC lines at passage 10 (a) and passage 20 (b) compared with the porcine embryonic fibroblasts (PEFs), which served as starting material (n = 3). Mean+ s.e.m. with asterisk* indicates statistically significant differences (p < 0.05).