Table 1.
Gene | Species | Sequence 5´ – 3’ | Product size (bp) | Reference No. (NCBI) |
---|---|---|---|---|
OCT4 | Porcine | 5´- AGGTGTTCAGCCAAACGACC −3’ 5´- TGATCGTTTGCCCTTCTGGC −3’ |
335 | NM_001113060.1 |
SOX2 | Porcine | 5´- CCAGAAGAACAGCCCAGA −3’ 5´- GCAGCGTTCGCAGCCG −3’ |
203 | NM_001123197.1 |
LIN28 | Porcine | 5´- CAGAGTAAGCTGCACATGGAGG −3’ 5´- GTAGGCTGGCTTTCCCTGTG −3’ |
325 | NM_001123133.1 |
NANOG | Porcine | 5´- CGAATGAAATGTAAGAGGT −3’ 5´- CCAGCTCTGATTACCCCAC −3’ |
162 | NM_001129971.1 |
OCT3/4 (pla) | Human | 5´- CATTCAAACTGAGGTAAGGG −3’ 5´- TAGCGTAAAAGGAGCAACATAG −3’ |
124 | Addgene plasmid #27077 |
SOX2 (pla) | Human | 5´- TTCACATGTCCCAGCACTACCAGA −3’ 5´- TTTGTTTGACAGGAGCGACAAT −3’ |
114 | Addgene plasmid #27078 |
KLF4 (pla) | Human | 5´- CCACCTCGCCTTACACATGAAGA −3’ 5´- TAGCGTAAAAGGAGCAACATAG −3’ |
156 | Addgene plasmid #27078 |
LIN28 (pla) | Human | 5´- AGCCATATGGTAGCCTCATGTCCGC −3’ 5´- TAGCGTAAAAGGAGCAACATAG −3’ |
251 | Addgene plasmid #27080 |
GAPDH | Porcine | 5´- TCGGAGTGAACGGATTTG −3’ 5´- CCTGGAAGATGGTGATGG −3’ |
219 | NM_001206359.1 |
GAPDH* | Porcine | 5´- TCGGAGTGAACGGATTTG −3’ 5´- CCTGGAAGATGGTGATGG −3’ |
158 | NC_010447.4 |
*GAPDH primers were specific for porcine genomic DNA.