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. 2018 Dec 3;17(23):2547–2563. doi: 10.1080/15384101.2018.1548790

Table 1.

Porcine- and plasmid-specific oligonucleotides.

Gene Species Sequence 5´ – 3’ Product size (bp) Reference No. (NCBI)
OCT4 Porcine 5´- AGGTGTTCAGCCAAACGACC −3’
5´- TGATCGTTTGCCCTTCTGGC −3’
335 NM_001113060.1
SOX2 Porcine 5´- CCAGAAGAACAGCCCAGA −3’
5´- GCAGCGTTCGCAGCCG −3’
203 NM_001123197.1
LIN28 Porcine 5´- CAGAGTAAGCTGCACATGGAGG −3’
5´- GTAGGCTGGCTTTCCCTGTG −3’
325 NM_001123133.1
NANOG Porcine 5´- CGAATGAAATGTAAGAGGT −3’
5´- CCAGCTCTGATTACCCCAC −3’
162 NM_001129971.1
OCT3/4 (pla) Human 5´- CATTCAAACTGAGGTAAGGG −3’
5´- TAGCGTAAAAGGAGCAACATAG −3’
124 Addgene plasmid #27077
SOX2 (pla) Human 5´- TTCACATGTCCCAGCACTACCAGA −3’
5´- TTTGTTTGACAGGAGCGACAAT −3’
114 Addgene plasmid #27078
KLF4 (pla) Human 5´- CCACCTCGCCTTACACATGAAGA −3’
5´- TAGCGTAAAAGGAGCAACATAG −3’
156 Addgene plasmid #27078
LIN28 (pla) Human 5´- AGCCATATGGTAGCCTCATGTCCGC −3’
5´- TAGCGTAAAAGGAGCAACATAG −3’
251 Addgene plasmid #27080
GAPDH Porcine 5´- TCGGAGTGAACGGATTTG −3’
5´- CCTGGAAGATGGTGATGG −3’
219 NM_001206359.1
GAPDH* Porcine 5´- TCGGAGTGAACGGATTTG −3’
5´- CCTGGAAGATGGTGATGG −3’
158 NC_010447.4

*GAPDH primers were specific for porcine genomic DNA.