Figure 3.

Phosphorylation of H2AX on serine 139 following Etoposide-induced DNA damage in U2OS cell lines knocked-down for MCM2 or MCM3. U2OS cells knocked-down with either a control shRNA, or shRNAs targeting MCM2 or MCM3 were treated or not for 1 hour (a) or 19 hours (b) with 1 or 10 µM Etoposide. Equal amounts of total cell lysates were separated by SDS-PAGE, and the proteins revealed by immunoblotting using the indicated antibodies. The ratio compared to the control and normalized with GAPDH is shown under each band, where applicable.