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Quantification of S-phase by EdU labeling. 293 and U2OS cells expressing shRNA for MCM2 and MCM3 were incubated with EdU for 2 h, followed by detection of EdU-DNA by Click-iT chemistry (Alex Fluor 488). Fluorescence was measured by FACS analysis by counting 100,000 cells. The experiment was repeated 3 times (n = 3) for each condition.
