Antibodies |
Sheep anti-CGRP |
Abcam |
Cat# ab22560 |
Rabbit anti-NF200 |
Sigma Aldrich |
Cat# N4142 |
Mouse anti-NF200 |
Sigma Aldrich |
Cat# N0142 |
Rabbit anti-TH |
Millipore |
Cat# AB152 |
Mouse anti-Parvalbumin |
Millipore |
Cat# MAB1572 |
Rabbit anti-PGP9.5 |
UltraClone Limited |
Cat# RA95101 |
Goat anti-TRPV1 |
Santa Cruz Biotechnology |
Cat# sc-12489 |
Rabbit anti-Substance P |
ImmunoStar |
Cat# 20064 |
Rabbit anti-RFP |
Rockland Antibodies & Assays |
Cat# 600-401-379S |
Rabbit anti-Trpm8 |
gift from M.Tominga, Okazai Institute for Integrative Bioscience |
|
Bacterial and Virus Strains |
AAV9.CAGGS.FLEX.ChR2-tdtomato.WRPE.SV40 |
Penn Vector Core |
Addgene18917 |
Chemicals, Peptides, and Recombinant Proteins |
Isolectin B4 (BSI-B4), biotin conjugate |
Sigma Aldrich |
Cat# L2140 |
Dynorphin A |
Tocris Biosciences |
Cat# 3195 |
nor-Binaltorphimine dihydrochloride |
Tocris Biosciences |
Cat# 0347 |
ICI204,488 hydrochloride |
Tocris Biosciences |
Cat# 0822 |
Naloxone hydrochloride |
Tocris Biosciences |
Cat# 0599 |
Nalfurafine |
Adooq Bioscience |
Cat# A12579 |
FE200665 |
from F. Porreca, University of Arizona |
|
RNAscope Probe- Mm-Oprk1 |
Advanced Cell Diagnostics |
Cat# 316111 |
RNAscope Probe- tdTomato-C2 |
Advanced Cell Diagnostics |
Cat# 317041-c2 |
Critical Commercial Assays |
RNAscope Multiplex Fluorescent Assay |
Advanced Cell Diagnostics |
Cat# 320850 |
MessageBOOSTER cDNA Sythesis Kit from Cell Lysates Kit |
Epicentre |
Cat# MBCL90310 |
RNA Clean & Concentrator-5 |
Zymo Research |
Cat# R1015 |
SsoAdvanced Universal SYBR Green Supermix |
BioRad |
Cat# 1725270 |
RNAscope Multiplex Fluorescent Assay |
Advanced Cell Diagnostics |
Cat# 320850 |
Experimental Models: Organisms/Strains |
Mouse: wild type C57BL/6 |
Charles River |
Strain Code 027 |
Mouse: KOR-cre
|
Cai et al., 2016 |
|
Mouse: Ai9 Rosa26-LSL-tdTomato
|
The Jackson Laboratory |
Stock No 007905 |
Mouse: Ai32 Rosa26-LSL-ChR2/EYFP
|
The Jackson Laboratory |
Stock No 024109 |
Other |
|
|
Nikon Elements Advanted Research |
Nikon |
https://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research |
R |
The R Project for Statistical Computing |
https://www.r-project.org/ |
GraphPad Prism 7 |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/ |
pClamp 10 |
Molecular Devices |
https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
Clampfit |
Molecular Devices |
https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
Signal 6 |
Cambridge Electronic Design |
http://ced.co.uk/products/sigovin |
Spike 2 |
Cambridge Electronic Design |
http://ced.co.uk/products/spkovin |
Excel |
Microsoft |
https://products.office.com/en-us/excel |
Illustrator CS5.1 |
Adobe |
https://www.adobe.com/creativecloud.html?promoid=KLXLR |
Photoshop CS5.1 |
Adobe |
https://www.adobe.com/creativecloud.html?promoid=KLXLR |