KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Sheep anti-CGRP | Abcam | Cat# ab22560 |
| Rabbit anti-NF200 | Sigma Aldrich | Cat# N4142 |
| Mouse anti-NF200 | Sigma Aldrich | Cat# N0142 |
| Rabbit anti-TH | Millipore | Cat# AB152 |
| Mouse anti-Parvalbumin | Millipore | Cat# MAB1572 |
| Rabbit anti-PGP9.5 | UltraClone Limited | Cat# RA95101 |
| Goat anti-TRPV1 | Santa Cruz Biotechnology | Cat# sc-12489 |
| Rabbit anti-Substance P | ImmunoStar | Cat# 20064 |
| Rabbit anti-RFP | Rockland Antibodies & Assays | Cat# 600-401-379S |
| Rabbit anti-Trpm8 | gift from M.Tominga, Okazai Institute for Integrative Bioscience | |
| Bacterial and Virus Strains | ||
| AAV9.CAGGS.FLEX.ChR2-tdtomato.WRPE.SV40 | Penn Vector Core | Addgene18917 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Isolectin B4 (BSI-B4), biotin conjugate | Sigma Aldrich | Cat# L2140 |
| Dynorphin A | Tocris Biosciences | Cat# 3195 |
| nor-Binaltorphimine dihydrochloride | Tocris Biosciences | Cat# 0347 |
| ICI204,488 hydrochloride | Tocris Biosciences | Cat# 0822 |
| Naloxone hydrochloride | Tocris Biosciences | Cat# 0599 |
| Nalfurafine | Adooq Bioscience | Cat# A12579 |
| FE200665 | from F. Porreca, University of Arizona | |
| RNAscope Probe- Mm-Oprk1 | Advanced Cell Diagnostics | Cat# 316111 |
| RNAscope Probe- tdTomato-C2 | Advanced Cell Diagnostics | Cat# 317041-c2 |
| Critical Commercial Assays | ||
| RNAscope Multiplex Fluorescent Assay | Advanced Cell Diagnostics | Cat# 320850 |
| MessageBOOSTER cDNA Sythesis Kit from Cell Lysates Kit | Epicentre | Cat# MBCL90310 |
| RNA Clean & Concentrator-5 | Zymo Research | Cat# R1015 |
| SsoAdvanced Universal SYBR Green Supermix | BioRad | Cat# 1725270 |
| RNAscope Multiplex Fluorescent Assay | Advanced Cell Diagnostics | Cat# 320850 |
| Experimental Models: Organisms/Strains | ||
| Mouse: wild type C57BL/6 | Charles River | Strain Code 027 |
| Mouse: KOR-cre | Cai et al., 2016 | |
| Mouse: Ai9 Rosa26-LSL-tdTomato | The Jackson Laboratory | Stock No 007905 |
| Mouse: Ai32 Rosa26-LSL-ChR2/EYFP | The Jackson Laboratory | Stock No 024109 |
| Other | ||
| Nikon Elements Advanted Research | Nikon | https://www.nikoninstruments.com/Products/Software/NIS-Elements-Advanced-Research |
| R | The R Project for Statistical Computing | https://www.r-project.org/ |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| pClamp 10 | Molecular Devices | https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
| Clampfit | Molecular Devices | https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
| Signal 6 | Cambridge Electronic Design | http://ced.co.uk/products/sigovin |
| Spike 2 | Cambridge Electronic Design | http://ced.co.uk/products/spkovin |
| Excel | Microsoft | https://products.office.com/en-us/excel |
| Illustrator CS5.1 | Adobe | https://www.adobe.com/creativecloud.html?promoid=KLXLR |
| Photoshop CS5.1 | Adobe | https://www.adobe.com/creativecloud.html?promoid=KLXLR |