Figure 6.

Knockdown of Sp1 reduces AA starvation-induced COX-2 expression. Indicated cells were subjected to AA starvation for 4 hours two days after transient transfection with siRNA against control (siC) or Sp1 (siSp1). COX-2 (A) protein and (B) mRNA expression were assessed. EIF5α and cyclophilin A again served as normalization controls. Blots are representative of three independent experiments. Graphs show the average value of three independent experiments with error bar representing ± one SEM. * and # indicate statistically significant (p < 0.05) difference by t-test compared with the corresponding initial and siC-transfected 4 hour time points, respectively.