FIGURE 4 |.

(A) Accumulative of soluble HA in the media over the course of GBM culture in GelMA hydrogels, measured via ELISA ( n = 3). GBM cells in GelMA hydrogels lacking any matrix immobilized HA (-HA) showed secreted significantly higher amount of soluble HA compare to GBM cells cultured within GelMA hydrogels containing matrix-bound HA. Production of soluble HA by GBM cells in GelMA hydrogels containing matrix-immobilized HA were strongly sensitive to the molecular weight of the matrix immobilized HA. Notably, soluble HA secretion increased with the MW of immobilized HA, with the 500 K group secreting significantly higher amount of soluble HA compare to 60 and 10 K. ∧p < 0.05 significant increase between different groups. (B) Agarose gel showing the molecular weight distribution of HA in culture media (blue). Low molecular weight chondroitin sulfate appears in purple. The most intense signals are samples at day 3 for 10, 60, and 500 K hydrogels.