Skip to main content
. 2018 Dec 19;13(12):e0208345. doi: 10.1371/journal.pone.0208345

Fig 6. Characterization of the VLPs used for rabbit immunization.

Fig 6

VLPs were produced by transfection into 293F cells and VLPs were pelleted from the clarified supernatants. (A) VLPs were analyzed by immunocrioelectromicroscopy labelling with an anti-gp120 antibody, 447-52D. (B) Envelope incorporations in AC10_29 and LR1-C1-VLPs were evaluated by the gp120 and p55 ratios by western blotting in SDS-PAGE gels using 447-52D and p24 (24–4) monoclonal antibodies. (C) Relative Env incorporation into VLPs was calculated by the ratios of gp120 to p55 using phosphorimaging analysis in triplicate. (D) The presence of trimers into AC10_29 and LR1-C1-VLPs and AC10_29 and LR1-C1-virions were analyzed by western blotting in BN-PAGE gels. The different forms of Env were labelled using a anti-gp120 and anti-gp41 cocktail (2G12, b12, 39F, 2F5, 4E10 and 447-52D). As controls, a 96ZM 651_HXB2_V3 SOSIP and a rgp120 were used.