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. 2018 Oct 17;4(12):4311–4320. doi: 10.1021/acsbiomaterials.8b00894

Figure 2.

Figure 2

The effect of 3D spheroid culture on gene transcription of SGSCs in floating, Matrigel, and microwell cultures. The mRNA levels of pluripotent stem cell markers (POU5F1, SOX2, and NANOG), salivary stem/progenitor cell markers (LGR5, THY1, ITGB1, HAS, and KRT5), salivary acinar markers (AMY1A and AQP5), and ductal cell markers (KRT7 and KRT18) were tested by real-time PCR, and the expression levels relative to GAPDH were calculated in triplicate after 1, 3, 5, and 7 days of culture. The data from three independent experiments were analyzed and presented as the mean ± standard errors of the mean (n = 9). Two-way ANOVA, Bonferroni posthoc test: *compared with 1-day group; #compared with 3-day group; $compared with 5-day group. *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001, $P < 0.05, $$P < 0.01, $$$P < 0.001.